Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction

Type of Document Master's Thesis
Author Lo, Terry
Author's Email Address tlo
URN etd-71497-17302
Title Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction
Degree Master of Science
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Dr. Inzana Committee Chair
Eric A. Wong none
Nammalwar Sriranganathan none
Keywords

* PCR
* multiplex
* serotyping
* Actinobacillus pleuropneumoniae

Date of Defense 1997-07-31
Availability unrestricted
Abstract

Traditional serologic assays of Actinobacillus pleuropeumoniae often have

problems with cross-reactivity. To avoid the complications of antibody-antigen

reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae

and identify serotype 5 strains. Primers specific to the conserved capsular export

region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the

exception of serotype 4. A second set of primers specific to the unique capsular

biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5

only. The sensitivity of this assay was determined to be less than 100 colony

forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively

distinguishes serotype 5 strains from other serotyes.

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