by Alan S. Gerstein
Product details
Paperback: 596 pages
Publisher: John Wiley & Sons Inc (12 Oct 2001)
Language English
ISBN: 0471379727
Size - 2.69 MB
Format PDF
Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as: Have you selected the most appropriate research strategy? Have you identified the issues critical to your successful application of a technique? Are you familiar with the limitations of a given technique? When should common procedural rules of thumb not be applied? What strategies could you apply to resolve a problem? A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book’s expert contributors include: Working safely with biological samples and radioactive materials; DNA and RNA purification; PCR; Protein and nucleid acid hybridisation; Prokaryotic and eukaryotic expression systems; Properly using and maintaining laboratory equipment.
• Does a company test every application for a product? • Is protein present in your RNA preparation and if so, should you be concerned? • Is it possible to determine the molecular weight of a polynucleotide? • What strategies should be used to optimize protein sample preparation for electrophoresis? • What are the critical considerations in planning a eukaryotic expression experiment?
If these questions sound familiar to you, this book should be on your shelf.
Molecular Biology Problem Solver: A Laboratory Guide will strengthen your ability to prevent, identify, and solve problems in the lab by teaching you to identify critical elements of any technique, reagent, or procedure. Rather than a collection of protocols, this guide is designed to help anyone from technicians to seasoned researchers obtain the tools needed to handle difficulties in the laboratory and prevent future ones.
Includes contributions from researchers at Ambion, Amersham Pharmacia Biotech, Bio Rad, Biochrom, Merck, New England Biolabs, Roche,as well as other companies and academic institutions.
Major issues explored by the book’s expert contributors include: • Working safely with biological samples and radioactive materials • DNA and RNA purification • Properly using and maintaining laboratory equipment • Prokaryotic and eukaryotic expression systems • Protein and nucleic acid hybridization • PCR • Electrophoresis • and much more
free medical book
No comments:
Post a Comment