by Kursad Turksen (Editor)
Product Details
* Hardcover: 385 pages
* Publisher: Humana Press; Bk&CD-Rom edition (March 31, 2006)
* Language: English
* ISBN-10: 1588294978
* ISBN-13: 978-1588294975
Table of Contents
ONTENTS
Preface
Contributors
Color Plates
1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells
Marsha L. Roach and John D. McNeish
2 The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos,
Gabriele Proetzel and Michael V. Wiles
3 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
Pierre Savatier, Hélène Lapillonne, Ludmila Jirmanova, Luigi Vitelli, and Jacques Samarut
4 Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation
André-Patrick Arrigo and Patrick Mehlen
5 Effects of Altered Gene Expression on ES Cell Differentiation
Yong Fan and J. Richard Chaillet
6 Hypoxic Gene Regulation in Differentiating ES Cells
David M. Adelman and M. Celeste Simon
7 Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells
Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, and Tetsuro Takamatsu
8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development
Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, and Shin-Ichi Nishikawa
9 Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System
Takumi Era, Stephane Wong, and Owen N. Witte
10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
Toshiyuki Yamane, Takahiro Kunisada, and Shin-Ichi Hayashi
11 Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells
Christian Dani
12 Embryonic Stem Cell Differentiation and the Vascular Lineage
Victoria L. Bautch
13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation
Anna M. Wobus, Kaomei Guan, Huang-Tian Yang, and Kenneth R. Boheler
14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications
Kishore B. S. Pasumarthi and Loren J. Field
15 Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
Jürgen Hescheler, Maria Wartenberg, Bernd K. Fleischmann, Kathrin Banach, Helmut Acker, and Heinrich Sauer
16 Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells
Tahmina Mujtaba and Mahendra S. Rao
17 Lineage Selection for Generation and Amplification of Neural Precursor Cells
Meng Li
18 Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson’s Disease
Hiroshi Kawasaki, Kenji Mizuseki, and Yoshiki Sasai
19 Epidermal Lineage
Tammy-Claire Troy and Kursad Turksen
20 ES Cell Differentiation Into the Hair Follicle Lineage In Vitro
Tammy-Claire Troy and Kursad Turksen
21 Embryonic Stem Cells as a Model for Studying Melanocyte Development
Toshiyuki Yamane, Shin-Ichi Hayashi, and Takahiro Kunisada
22 Using Progenitor Cells and Gene Chips to Define Genetic Pathways
S. Steven Potter, M. Todd Valerius, and Eric W. Brunskill
23 ES Cell-Mediated Conditional Transgenesis
Marina Gertsenstein, Corrinne Lobe and Andras Nagy
24 Switching on Lineage Tracers Using Site-Specific Recombination
Susan M. Dymecki, Carolyn I. Rodriguez, and Rajeshwar B. Awatramani
25 From ES Cells to Mice: The Gene Trap Approach
Francesco Cecconi and Peter Gruss
26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells
Thomas Floss and Wolfgang Wurst
27 Phage-Displayed Antibodies to Detect Cell Markers
Jun Lu and Steven R. Sloan
28 Gene Transfer Using Targeted Filamentous Bacteriophage
David Larocca, Kristen Jensen-Pergakes, Michael A. Burg, and Andrew Baird
29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors
Jane E. Aubin, Fina Liu, and G. Antonio Candeliere
30 Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays
Thorsten Hoevel and Manfred Kubbies
31 Expression Profiling Using Quantitative Hybridization on Macroarrays
Geneviève Piétu and Charles Decraene
32 Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells
Eric Tse, Grace Chung, and Terence H. Rabbitts
33 Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
Ingrid Remy, André Galarneau, and Stephen W. Michnick
34 Direct Selection of cDNAs by Phage Display
Reto Crameri, Gernot Achatz, Michael Weichel, and Claudio Rhyner
35 Screening for Protein–Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells
R. Daniel Gietz and Robin A. Woods
Index
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